In vitro evaluation of the capacity of zeolite and bentonite to adsorb aflatoxin B1 in simulated gastrointestinal fluids

Thieu N.Q., Pettersson H.
Department of Animal Nutrition, Nong Lam University, Linhtrung Ward Thuduc District Hochiminh City, Viet Nam; Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine and Animal Science, Uppsala, Sweden

Abstract: Anin vitro study using single concentration and isotherm adsorption was carried out to evaluate the capacity of Vietnamese produced zeolite and bentonite to adsorb aflatoxin B1 (AFB1) in simulated gastrointestinal fluids (SGFs), and a commercial sorbent hydrated sodium calcium aluminosilicate (HSCAS) was used as reference. In this study, AFB1 solution was mixed with sorbents (0.3, 0.4 and 0.5% w/v) in SGFs at pH 3 and pH 7 and shaken for 8 h, centrifuged and the supernatant measured by Vicam fluorometer. Adsorption of AFB1 onto zeolite and bentonite varied according to the pH of SGFs and was lower than HSCAS. Linearity between the increased amount of AFB1 adsorbed on sorbents and the decrease of sorbent concentration was observed for bentonite and HSCAS, except for zeolite in SGFs at pH 7. The observed maximum amounts of AFB1 adsorbed on bentonite and HSCAS were 1.54 and 1.56 mg/g, respectively. The adsorption capacities of bentonite and HSCAS for AFB1 were 12.7 and 13.1 mg/g, respectively, from fitting the data to the Freundlich isotherm equation. Improvement in processing and purification for bentonite is needed to enhance the surface area, which would probably result in better adsorptive capacity for this sorbent. © 2008 Society of Mycotoxin Research and Springer.
Author Keywords: Aflatoxin B1; Bentonite; HSCAS; Simulated gastrointestinal fluids; Zeolite
Index Keywords: aflatoxin B1; aluminosilicate calcium; bentonite; hydrated sodium calcium aluminosilicate; sorbent; unclassified drug; zeolite; absorption; adsorption; article; centrifugation; concentration (parameters); fluorometer; in vitro study; isotherm; pH; stomach juice; supernatant

Year: 2008
Source title: Mycotoxin Research
Volume: 24
Issue: 3
Page : 124-129
Link: Scorpus Link
Authors with affiliations:

1. Thieu, N.Q., Department of Animal Nutrition, Nong Lam University, Linhtrung Ward Thuduc District Hochiminh City, Viet Nam
2. Pettersson, H., Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine and Animal Science, Uppsala, Sweden

Correspondence Address: Thieu, N. Q.; Department of Animal Nutrition, Nong Lam University, Linhtrung Ward Thuduc District Hochiminh City, Viet Nam; email: qthieu68@yahoo.com
Download Abstract: HN_U682.pdf