Detection of cystic fibrosis related DNA targets using AC field focusing of magnetic labels and spin-valve sensors

Ferreira H.A., Graham D.L., Feliciano N., Clarke L.A., Amaral M.D., Freitas P.P.
IEEE; INESC-Microsystems and Nanotechnologies, Lisbon, Portugal; Physics Department, Instituto Superior Técnico, Technical University of Lisbon, Lisbon, Portugal; Chemistry and Biochemistry Department, Faculty of Sciences, University of Lisbon, Lisbon, Portugal; Centre for Human Genetics, National Institute of Health, Lisbon, Portugal

Abstract: A spin-valve sensor biochip was used to detect cystic fibrosis related DNA targets for the purpose of developing an affordable diagnostic chip. The strategy used was based on the ac field focusing of magnetically labeled target DNA at sensor sites using U-shaped current lines. U-shaped spin-valve sensors, fabricated within the line structures, detected in real-time the hybridization of DNA targets to complementary DNA probes, previously immobilized onto the chip surface. Hybridization occurred in relatively short times (15-30 min) in comparison with conventional hybridization approaches (3 to 12 h). Statistical data on detection signals for single probe and multiprobe experiments was obtained, showing a significant difference between complementary binding signals and noncomplementary and background ones. © 2005 IEEE.
Author Keywords: Biomedical transducers; DNA; Magnetic particles; Magnetoresistive devices; Medical diagnosis

Year: 2005
Source title: IEEE Transactions on Magnetics
Volume: 41
Issue: 10
Page : 4140-4142
Cited by: 22
Link: Scorpus Link
Document Type: Conference Paper
Source: Scopus
 Authors with affiliations:
  1. Ferreira, H.A., IEEE, INESC-Microsystems and Nanotechnologies, Lisbon, Portugal, Physics Department, Instituto Superior Técnico, Technical University of Lisbon, Lisbon, Portugal
  2. Graham, D.L., INESC-Microsystems and Nanotechnologies, Lisbon, Portugal
  3. Feliciano, N., INESC-Microsystems and Nanotechnologies, Lisbon, Portugal
  4. Clarke, L.A., Chemistry and Biochemistry Department, Faculty of Sciences, University of Lisbon, Lisbon, Portugal
  5. Amaral, M.D., Chemistry and Biochemistry Department, Faculty of Sciences, University of Lisbon, Lisbon, Portugal, Centre for Human Genetics, National Institute of Health, Lisbon, Portugal
  6. Freitas, P.P., INESC-Microsystems and Nanotechnologies, Lisbon, Portugal, Physics Department, Instituto Superior Técnico, Technical University of Lisbon, Lisbon, Portugal
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