Ultrasensitive detection of nanoparticle-based bio bar-code DNA sensor by using nano-gap electrodes

Chang T.-L., Tsai C.-Y., Uppala R., Chen C.-C., Lin C.-H., Chen P.-H.
Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan; National Nano Device Laboratories, Hsinchu 300, Taiwan

Abstract: An ultra sensitive approach to detect the sub femtomolar DNA by using electrical detection method on a DNA biochip has been developed. The system provides high selectivity with sensitivity which is comparable to nano-gap-electrode detection approaches without the need of PCR amplification. Because bio-bar-code DNA approach is a pseudohomogeneous system with both magnetic nanoparticles and gold nanoparticles in solution, low concentration of the probes can be used very efficiently to bind target DNA, thereby reducing time required for high sensitivity detection experiments. Indeed, an advantage of the bio-bar-code DNA approach over conventional microarray sandwich assembly assays is that the entire self-assembly assay can be carried out in sub femtomolar concentration, regardless of target concentration. The system has an excellent dynamic range and is ideally set up for multiplexing.
Author Keywords: Bio-bar-code DNA detection; DNA biochip; Electrical detection; Gold nanoparticles; Magnetic nanoparticles; Self-assembly

Year: 2005
Source title: Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
Volume: 2005
Art. No.: 1595288
Page : 210-211
Link: Scorpus Link
Document Type: Conference Paper
Source: Scopus
 Authors with affiliations:
  1. Chang, T.-L., Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
  2. Tsai, C.-Y., Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
  3. Uppala, R., Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
  4. Chen, C.-C., National Nano Device Laboratories, Hsinchu 300, Taiwan
  5. Lin, C.-H., National Nano Device Laboratories, Hsinchu 300, Taiwan
  6. Chen, P.-H., Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
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