Giant magnetoresistive biosensors for molecular diagnosis: Surface chemistry and assay development

Yu H., Osterfeld S.J., Xu L., White R.L., Pourmand N., Wang S.X.
MagArray Inc., 1230 Bordeaux Dr., Sunnyvale, CA 94806, United States; Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States; Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States

Abstract: Giant magnetoresistive (GMR) biochips using magnetic nanoparticle as labels were developed for molecular diagnosis. The sensor arrays consist of GMR sensing strips of 1.5 μm or 0.75 μm in width. GMR sensors are exquisitely sensitive yet very delicate, requiring ultrathin corrosion-resistive passivation and efficient surface chemistry for oligonucleotide probe immobilization. A mild and stable surface chemistry was first developed that is especially suitable for modifying delicate electronic device surfaces, and a practical application of our GMR biosensors was then demonstrated for detecting four most common human papillomavirus (HPV) subtypes in plasmids. We also showed that the DNA hybridization time could potentially be reduced from overnight to about ten minutes using microfluidics.
Author Keywords: Biochip; DNA microarray; Giant magnetoresistance; HPV; Immobilization; Surface chemistry

Year: 2008
Source title: Proceedings of SPIE - The International Society for Optical Engineering
Volume: 7035
Art. No.: 70350E
Cited by: 1
Link: Scorpus Link
Document Type: Conference Paper
Source: Scopus
 Authors with affiliations:
  1. Yu, H., MagArray Inc., 1230 Bordeaux Dr., Sunnyvale, CA 94806, United States
  2. Osterfeld, S.J., MagArray Inc., 1230 Bordeaux Dr., Sunnyvale, CA 94806, United States
  3. Xu, L., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States
  4. White, R.L., MagArray Inc., 1230 Bordeaux Dr., Sunnyvale, CA 94806, United States
  5. Pourmand, N., Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States
  6. Wang, S.X., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States
Download Abstract: biochips30.pdf