Multiplex protein assays based on real-time magnetic nanotag sensing

Osterfeld S.J., Yu H., Gaster R.S., Caramuta S., Xu L., Han S.-J., Hall D.A., Wilson R.J., Sun S., White R.L., Davis R.W., Pourmand N., Wang S.X.
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States; Department of Bioengineering, Stanford University, Stanford, CA 94305, United States; Department of Electrical Engineering, Stanford University, Stanford, CA 94305, United States; Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, United States; Department of Chemistry, Brown University, Providence, RI 02912, United States; Biomolecular Engineering, University of California, 1156 High Street, Santa Cruz, CA 95064, United States

Abstract: Magnetic nanotags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, because minute quantities of MNTs can be detected with inexpensive giant magnetoresistive (GMR) sensors, such as spin valve (SV) sensors. However, translating this promise into easy to use and multilplexed protein assays, which are highly sought after in molecular diagnostics such as cancer diagnosis and treatment monitoring, has been challenging. Here, we demonstrate multiplex protein detection of potential cancer markers at subpicomolar concentration levels and with a dynamic range of more than four decades. With the addition of nanotag amplification, the analytic sensitivity extends into the low fM concentration range. The multianalyte ability, sensitivity, scalability, and ease of use of the MNT-based protein assay technology make it a strong contender for versatile and portable molecular diagnostics in both research and clinical settings. © 2008 by The National Academy of Sciences of the USA.
Author Keywords: Biochip; Giant magnetoresistive sensors; GMR; Magnetic nanotags; Multiplex protein detection

Year: 2008
Source title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 105
Issue: 52
Page : 20637-20640
Cited by: 31
Link: Scorpus Link
Document Type: Article
Source: Scopus
Authors with affiliations:

1. Osterfeld, S.J., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States
2. Yu, H., Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, United States
3. Gaster, R.S., Department of Bioengineering, Stanford University, Stanford, CA 94305, United States
4. Caramuta, S., Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, United States
5. Xu, L., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States
6. Han, S.-J., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States
7. Hall, D.A., Department of Electrical Engineering, Stanford University, Stanford, CA 94305, United States
8. Wilson, R.J., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States
9. Sun, S., Department of Chemistry, Brown University, Providence, RI 02912, United States
10. White, R.L., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States, Department of Electrical Engineering, Stanford University, Stanford, CA 94305, United States
11. Davis, R.W., Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, United States
12. Pourmand, N., Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, United States, Biomolecular Engineering, University of California, 1156 High Street, Santa Cruz, CA 95064, United States
13. Wang, S.X., Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States, Department of Electrical Engineering, Stanford University, Stanford, CA 94305, United States

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